One milliliter of butanol-pyridine 15:1 (v/v) was then added to each sample, which was vortexed and centrifuged at 20,000 × g for 5 minutes. The absorbances of the supernatants (the butanol-pyridine ...

3. A total volume of 200 μl of APL2 buffer was added. The lid was closed, and the samples were vortexed for 30 s. 4. Samples were incubated at 70 °C for 10 min. 5. The tubes were briefly spun. 6.

LOS ANGELES — Antifungal therapy relieves symptoms in patients with severe asthma, chronic sinusitis, or both whose sputum tests positive for fungal cultures, new research shows. The approach ...

How to talk to your polar-vortexed friends It's hard to bring up global warming (hint: say 'climate change') when it's really cold out, but here are some pointers. Shutterstock Anna Fahey Published ...