The reaction was performed using the 7500 Fast Real-Time PCR System (Applied Biosystems) as following procedure: (1) reverse transcription at 55°C for 15 min; (2) a pre-denaturation step at 95°C for ...

RT-PCR was carried out using Taqman Fast on a 7500 real-time PCR system (Applied Biosystems). Threshold cycles for each transcript were normalized to GAPDH (ΔCt). Calibrations and normalizations used ...

This is a valuable study on the efficacy of a live attenuated vaccine that was tested in different animal models. The evidence is convincing, but it could be further strengthened by comparing the ...

Real-time PCR was performed using the TB Green Premix Ex Taq II system (TaKaRa) with an Applied Biosystem 7500 instrument (Applied Biosystems).21 Primer sequences were listed in online supplemental ...

THE Cavite State University (CvSU) delegation composed of Bachelor of Science in Agricultural and Biosystems Engineering students emerged as champions in the 1st Philippine Society of Agriculture and ...

Trump’s Take On EV Tax Credits: In November, Reuters reported that Donald Trump‘s transition team plans to kill the $7,500 tax credit available on EV purchases. On Monday, after his ...

The removal of the $7500 federal tax credit on the purchase of electric vehicles will effectively raise the price of Tesla Inc TSLA vehicles in the U.S. as compared to its peers and pose a huge ...

PCR tests represent a major advance in the rapid diagnosis of infectious diseases. The qPCR technology has allowed easier and more effective implantation of molecular diagnosis in the clinical ...