Even as it passes its 40 th birthday 1, polyacrylamide gel electrophoresis ... “With 2-D gels,” says Garrels, “you've got the chemistry, the vagaries of the gel, the solubilization conditions ...

Large protein complexes greater than 1 MDa in mass are difficult to resolve and migrate quite slowly in the native polyacrylamide gels. For analyzing larger complexes, use either agarose or ...