Biotinylated nucleotides have emerged as a powerful tool in molecular biology, enabling researchers to explore genetic materials with unprecedented precision and sensitivity. These modified ...

Researchers from Waters and Biospring studied the contribution of nucleotide type and modifications on the retention and resolution of 22–24 nt long oligonucleotides in different chromatographic ...

With continued research, modified HDOs could pave the way for a new generation of drugs that effectively target brain diseases, offering hope to millions of patients and their families around the ...

A significant breakthrough in RNA therapeutics, this self-amplifying technology overcomes key challenges by incorporating alternative chemical building blocks. Detailed in a Nature Biotechnology ...

We next asked whether the reduction and depurination steps in BASH MaP impaired detection of other methylated nucleotides that are formed in single-stranded RNA. Of the five DMS-modified nucleotides, ...

Reference: “Complete substitution with modified nucleotides in self-amplifying RNA suppresses the interferon response and increases potency” by Joshua E. McGee, Jack R. Kirsch, Devin Kenney, Faith ...

Modified oligonucleotides-based therapies involve the use of chemically-modified RNAi constructs, antisense compounds, and siRNA to target and inhibit the expression of specific genes associated with ...

To solve this issue, they modified their nucleotides with a "blocker," a chemical group that stalls the enzyme and only allows for the addition of one nucleotide at a time.

To solve this issue, they modified their nucleotides with a "blocker," a chemical group that stalls the enzyme and only allows for the addition of one nucleotide at a time.

Furthermore, the researchers modified the nucleotides by attaching a chemical group called a “blocker” that ensures that nucleotides are added to the RNA chain one at a time.