The supernatant was measured for IFN-γ using the ClinMax ™ Human IFN-γ ELISA kit. These measurements revealed that NK cells co-cultured with K562 secreted greater levels of IFN-γ than the ...

Macrophages from wild-type or Stat1 KO mice were incubated in the presence or absence of IFN-γ for 60 hours, at which time, supernatants were assayed for interleukin-6 (IL-6) levels by ELISA ...

IFN-γ mRNA was detected by reverse transcriptase polymerase chain reaction, and protein secretion was measured by enzyme linked immunosorbent assay (ELISA). Conclusions—IL-12 induces human T-LPLs to ...

Fontolizumab (HuZAF) is the humanised form of a murine antihuman IFN-γ antibody (Protein Design Labs ... Screening for circulating antibodies to fontolizumab was performed using a bridging ELISA.

Memorial Sloan Kettering Cancer Center investigators report that T cell interferon-γ (IFNγ) orchestrates a dendritic-cell–natural-killer-cell circuit that suppresses leptomeningeal metastases in mouse ...

Background: Fatigue and impaired performance in athletes is well recognised and has been loosely linked to “overtraining”. Reduced concentration of IgA in the saliva and increased shedding of Epstein ...

mondino.it Intracellular cytokine flow cytometry was used to analyse the percentages of interferon (IFN) γ and interleukin (IL)-4 producing T cells in the peripheral blood of multiple sclerosis ...

Indeed, the current study is built upon the foundational work of Zhang et al. (PMID: 23175475), which first described the proinflammatory IFN-γ + phenotype of CD4 T cells in CD11c-p28 floxed mice ...

Objective: Interferon-γ release assay (IGRAs) promised a sensitive and specific diagnosis of latent and active infection of Mycobacterium tuberculosis(M.tb). Therefore, the intent of this study was to ...

The newly generated CD4 single-positive (SP) T lymphocytes are featured by enhanced IL-4 but repressed IFN-γ production. The mechanisms underlying this functional bias remain elusive. Previous studies ...