Chromatin immunoprecipitation sequencing (ChIP-seq), assay for transposase-accessible chromatin sequencing (ATAC-seq), and cleavage under targets and tagmentation (CUT&Tag) assays were performed ...

Methods Gastrointestinal cell lines were co-transfected with wild-type or mutated Cdx2 promoter constructs and CDX2 expression vector for luciferase assays ... qPCR and western blotting. Chromatin ...

Primary microglial cells were treated with OGD and FTY720, and Chromatin immunoprecipitation (ChIP) assay was performed to evaluate the acetylation levels of histone H3 and H4 binding to the Sp1 in ...

We find that multiple Shu complex members interact with the ORC complex by yeast-2-hybrid (Y2H) and co-immunoprecipitation (coIP), and these interactions occur in G1 in a DNA-independent manner. The ...

Using the coimmunoprecipitation (Co-IP) assay, we verified that the ... deletion of double copies of ICP34.5 gene) in Vero cells. ChIP analysis was conducted using the SimpleChIP Enzymatic Chromatin ...

Objective The HBV HBx regulatory protein is required for transcription from the covalently closed circular DNA (cccDNA) minichromosome and affects the epigenetic control of both viral and host ...

For cell viability in normal culture, cells were cultured for 96h, and MTS-based cell viability was measured at 0h, 24h, 48h, 72h, and 96h using Cell Viability Colorimetric Assay Kit (Promega).

Using the coimmunoprecipitation (Co-IP) assay, we verified that the ... values were calculated using the 2 −ΔΔCt method. ChIP analysis was conducted using the SimpleChIP Enzymatic Chromatin IP kit ...

aDivision of Life Science, Daniel and Mayce Yu Molecular Neuroscience Center, State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science and Technology, Hong Kong Special ...