Frontiers5d
The Intra-nuclear SphK2-S1P Axis Facilitates M1-to-M2 Shift of Microglia via Suppressing HDAC1-Mediated KLF4 Deacetylation
Primary microglial cells were treated with OGD and FTY720, and Chromatin immunoprecipitation (ChIP) assay was performed to evaluate the acetylation levels of histone H3 and H4 binding to the Sp1 in ...
Frontiers5d
ATF5 promotes malignant T cell survival through the PI3K/AKT/mTOR pathway in cutaneous T cell lymphoma
For cell viability in normal culture, cells were cultured for 96h, and MTS-based cell viability was measured at 0h, 24h, 48h, 72h, and 96h using Cell Viability Colorimetric Assay Kit (Promega).
gut.bmj5d
Single-cell RNA-seq analysis reveals BHLHE40-driven pro-tumour neutrophils with hyperactivated glycolysis in pancreatic tumour microenvironment
and direct transcriptional regulation of BHLHE40 on TAN-1 marker genes was demonstrated by chromatin immunoprecipitation assay. Pro-tumour and immunosuppression functions were observed in dHL-60 cells ...
gut.bmj5d
Inflammatory cell-derived CXCL3 promotes pancreatic cancer metastasis through a novel myofibroblast-hijacked cancer escape mechanism
Objective Pancreatic ductal adenocarcinoma (PDAC) is the most lethal malignancy and lacks effective treatment. We aimed to understand molecular mechanisms of the intertwined interactions between ...