The universal P RT-qPCR primer-probe set detected all hMPV clinical isolates across all four subgroups. Virus quantification for TN/1501/A1, TN/94-49/A2, and TN/98-242/B1 strains were successfully ...

Reverse Transcription Polymerase Chain Reaction (RT-PCR) is the gold standard diagnostic tool for detecting HMPV RNA, while antigen detection assays offer quicker results. In India, surveillance ...

This RT-PCR assay was performed with the primer ... Retrospective studies have shown the presence of human metapneumovirus (HMPV) in the human population from as early as the 1950s.

The perceived ease of use of real-time quantitative PCR (qPCR) and reverse transcription PCR (RT-qPCR) technology has revolutionized life science research. Its effectiveness at amplification and ...

Using reverse transcription polymerase chain reaction (RT-PCR), they screened for respiratory pathogens, including hMPV, influenza, and respiratory syncytial virus. Additionally, during an ...

The GoTaq ® Probe 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and ...

5. Like all forms of PCR, qPCR and RT-qPCR requires optimization to ensure sufficient and specific amplification. You may need to optimize PCR parameters such as magnesium concentration ...

Variation in gene expression among cell types, tissues, and organisms is commonly examined by reverse transcription quantitative PCR, or RT-qPCR. In this process, RNA is isolated from samples of ...

Real-time PCR, or quantitative qPCR, is a commonly used molecular biology lab technique to determine the actual amount of PCR product at a given cycle. For quantitative reverse transcription PCR ...

To help boost adoption, Bio-Rad scientists published a brief and practical guide that concisely summarizes the key steps required to produce high-quality, reproducible data for labs conducting RT-qPCR ...