RNA immunoprecipitation experiments showed an essential role of RRM1 in recognizing all tested targets, while the dsRBD only plays a role in binding a subset. The affinity of RRM1 for a single ...

Using RNA and DNA extraction ... Chromatrap ® FFPE ChIP kit protocol was followed for the preparation of the immunoprecipitation slurries. In each 1ml slurry, 20 µL of chromatin stock was ...