In the microscopic world of bacteria, gene transfer is a powerful mechanism that can alter cellular function, drive ...

In the microscopic world of bacteria, gene transfer is a powerful mechanism that can alter cellular function, drive antibiotic resistance and even shape entire ecosystems.

Conjugation is a form of horizontal gene transfer that allows the exchange of genetic material between two organisms or cells ...

However, plasmid copy numbers can fluctuate, causing inconsistent gene expression within bacterial populations. By balancing CRISPR-Cas-mediated cutting and gene transfer, ADEPT dynamically ...

Bacteria commonly produce toxins that are lethal to themselves, but also produce the required antitoxins. These toxin-antitoxin (TA) systems may be useful in modifying bacteria for biotechnology ...

This article discusses the mechanisms of bacterial transduction and how scientists can utilize it to manipulate bacterial ...

13 μg packaging plasmid pCMVΔR8.74 or pCMVΔR8.2 and 20 μg transfer plasmid (pHR2PGKNLSLacZ or pHR2EF1α cytoLacZ). For MLV, we used 27 μg plasmid: 7 μg pMD.G and 20 μg pLPGKNLSLacZ.