Biotinylated nucleotides have emerged as a powerful tool in molecular biology, enabling researchers to explore genetic materials with unprecedented precision and sensitivity. These modified ...

Interestingly, this extra RNA strand can be further modified by attaching a cholesterol (Chol) molecule to create Chol-HDOs. Building on recent reports about the enhanced ability of Chol-HDO to ...

By fully substituting the RNA with modified nucleotides, they successfully reduced inflammatory responses, resulting in enhanced performance of a COVID-19 vaccine with efficacy at 100X lower doses ...

A further drawback of these approaches is their limitations when modified nucleotides are used to decrease the immunogenicity or enhance the stability of these molecules (7). Mass spectrometry (MS) is ...

Reference: “Complete substitution with modified nucleotides in self-amplifying RNA suppresses the interferon response and increases potency” by Joshua E. McGee, Jack R. Kirsch, Devin Kenney, Faith ...

Modified nucleotide therapies is a key innovation area in the pharmaceutical industry Modified oligonucleotides-based therapies involve the use of chemically-modified RNAi constructs, antisense ...

To solve this issue, they modified their nucleotides with a "blocker," a chemical group that stalls the enzyme and only allows for the addition of one nucleotide at a time.

To solve this issue, they modified their nucleotides with a "blocker," a chemical group that stalls the enzyme and only allows for the addition of one nucleotide at a time.

Furthermore, the researchers modified the nucleotides by attaching a chemical group called a “blocker” that ensures that nucleotides are added to the RNA chain one at a time. Once the desired ...

To carry out this method, the researchers feed cells modified nucleotides that contain a special alkyne group. During a burst of transcription, those modified nucleotides get incorporated into the ...