Figure 3: GFP expression in peripheral blood leukocytes ... Cells were transduced with a GFP-containing retroviral vector for 3.5 days on Retronectin- (Takara Biomedicals, Shiga, Japan) treated ...

Purify the vector products by electrophoresis through ... Poor expression of fusions. Check for expression of GFP fusions on SDS-PAGE. Vary concentrations of inducer to obtain roughly ...