After 10 days of exposure, the cells were loaded with mitochondrial superoxide sensor MitoSOX Red (2.5 μmol/L) and DNA stain Hoechst 33342 (10 μmol/L) for 25 min. Reading medium (PBS ...

We have conducted a phenotypic screening in endothelial cells exposed to elevated extracellular glucose (an in vitro model of hyperglycemia) to identify compounds that prevent hyperglycemia ...