The EMBO Journal18d
The Shu complex interacts with the replicative helicase to prevent mutations and aberrant recombination
We find that multiple Shu complex members interact with the ORC complex by yeast-2-hybrid (Y2H) and co-immunoprecipitation (coIP), and these interactions occur in G1 in a DNA-independent manner. The ...
News Medical26d
Short-chain fatty acids propionate and butyrate directly modify chromatin to regulate gene expression
Chromatin immunoprecipitation sequencing (ChIP-seq), assay for transposase-accessible chromatin sequencing (ATAC-seq), and cleavage under targets and tagmentation (CUT&Tag) assays were performed ...
icr.ac.uk1mon
Closed: Examining the mechanism of chromatin telomere replication
Experimental approaches will include chromatin immunoprecipitation assays and immunofluorescence to examine the level of histones and the appearance of DNA damage markers at telomeres. Does binding of ...
Frontiers2y
Vitamin D3 alleviates inflammation in ulcerative colitis by activating the VDR-NLRP6 signaling pathway
The SimpleChip Plus Enzymatic Chromatin IP Kit (Cat# 9004, Cell Signalling, China) was used in the ChIP assay with mouse clone tissues. Immunoprecipitation was ... to detect immunoreactive protein ...
Labroots8y
Studying Kinetics of Chromatin Assembly with SWATH-MS
The structure of chromatin is critical for many aspects of cellular physiology and is considered to be the primary medium to store epigenetic information. The nucleosomes together with the non-histone ...