The obtained data were analyzed using a comparison threshold cycle (2 −ΔΔCt) to indicate relative mRNA expression, and GAPDH was used as an ... calculated for each group. The chromatin ...

The solid data in mouse embryonic stem cells include separation of function mutations in IκBα to dissect its non-canonical role as a chromatin regulator and its ... CT of the housekeeping gene Tbp or ...

Our mission is to provide a state-of-the-art facility, expertise, and education in advanced quantitative light microscopy to support Drexel University’s research community The Cell Imaging Center (CIC ...

Expert Rev Mol Diagn. 2013;13(5):445-455. Dkk-3 was silenced or downregulated in 71% of gastric cancer cell lines. The transcriptional silencing was associated with promoter methylation, and it ...

Objective The HBV HBx regulatory protein is required for transcription from the covalently closed circular DNA (cccDNA) minichromosome and affects the epigenetic control of both viral and host ...

Using the coimmunoprecipitation (Co-IP) assay, we verified that the ... deletion of double copies of ICP34.5 gene) in Vero cells. ChIP analysis was conducted using the SimpleChIP Enzymatic Chromatin ...

and direct transcriptional regulation of BHLHE40 on TAN-1 marker genes was demonstrated by chromatin immunoprecipitation assay. Pro-tumour and immunosuppression functions were observed in dHL-60 cells ...

Mechanistically, we constructed an MYBL2 regulatory network in melanoma by integrating RNA-seq and ChIP-seq data. EPPK1 ... were determined by qPCR of MYBL2 mRNA and Western blot assays for MYBL2 ...

aDivision of Life Science, Daniel and Mayce Yu Molecular Neuroscience Center, State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science and Technology, Hong Kong Special ...