Methods: In this study, we integrated biochemical assays with a panel of cellular analyses ... through integrated network pharmacology approaches. Chromatin immunoprecipitation quantitative PCR ...

Antibodies against GAPDH (#AC002) was purchased from ABclonal Technology ... through ChampChemiTM (SageCreation). The chromatin immunoprecipitation (ChIP) assay was performed according to the ...

For experimental validation, ChIP-quantitative PCR was performed at the promoters ... Two or 10 million cells were harvested for RNA extraction and chromatin immunoprecipitation assays, respectively.

Characterization of VGLL2-NCOA2- and YAP-controlled transcriptional and chromatin ... the immunoprecipitation and western blot following the protocols described previously (Cotton et al., 2017). The ...

A pinched nerve occurs when there is pressure or force on an area of a nerve, causing it to send pain signals to the brain. Most cases are mild, and a person can often treat them at home. In this ...

Epigenetic regulation orchestrated by changes in chromatin openness modulates behavioral plasticity by controlling gene expression. However, the gene regulation mechanisms by which chromatin openness ...

High nuclear accumulation of NONO-TFE3 or PRCC-TFE3 fusion proteins promotes their broad binding across the genome at H3K27ac-marked active chromatin, engaging a core ... identity and stimulates ...