Due to its conical design, the tube fits into all standard 15 ml centrifuge rotors and the cells can be sedimented directly within the culture tube. This eliminates the need for time-consuming ...

Mix and incubate cells at 37 °C overnight. The following day, transfer cells to a 15-ml centrifuge tube and spin at 1,500 r.p.m. (500g) for 5 min at 25 °C. Recover supernatant and filter it ...