This RT-PCR assay was performed with the primer sets ... Retrospective studies have shown the presence of human metapneumovirus (HMPV) in the human population from as early as the 1950s.

[15] Samples were analyzed in triplicate in three independent PCR assays. The viruses hMPV, PIV-1, PIV-2 and PIV-3 were quantified by LightMix assays (TIB MOLBIOL, Berlin, Germany). The virus ...