Researchers can insert DNA fragments or genes into a plasmid vector, creating a so-called recombinant plasmid. This plasmid can be introduced into a bacterium by way of the process called ...
Immunizing the host with a piece of viral DNA rather than an antigenic protein fragment of the virus, helps to stimulate the generation of cell-mediated immunity (Fig. 2). DNA vaccines contain the ...
The Gene Vector Core was awarded the Blue Flame Award. The award is given to scientists who have at least one plasmid deposited that has been distributed at least 100 times. The Gene Vector Core was ...
This cassette is then transferred to the adenovirus vector plasmid (pAdeno X, Clontech Laboratories or pAd5F35, The Journal of Gene Medicine Volume 6, Issue 6 , Pages 631 - 641). The adenovirus vector ...
The ‘transfer plasmid’ or lentiviral vector contains the transgene of interest, while the ‘envelope plasmid’ contains genes for proteins that appear on the virus surface, the ‘packaging ...
Note: for plasmid DNA the size is the entire plasmid, vector plus insert. For plasmid DNA you may use the “divide by 20 rule” where you divide the size of the plasmid by 20 to determine the nanograms ...