The miRNA portion of the duplex binds AGO1 to form RISC. The miRNA bound in RISC base pairs with a target mRNA that is complementary to the miRNA. The target mRNA is repressed by either cleavage ...

Only one strand of the miRNA:miRNA* duplex or the siRNA duplex is preferentially assembled into the RNA-induced silencing complex (RISC) , which subsequently acts on its target by translational ...

View Full Profile. Learn about our Editorial Policies. The natural RNAi mechanism can be directed by either short interfering RNA (siRNA) or microRNA (miRNA). 1 There are four key steps in the RNAi ...