Laser alignment is tricky, but we’ve seen a few other solutions out there. For a more classic mechanism that traces the actual beam path with burn marks, have a look at [Stephen’s] method.

Laser-induced alignment boosts single-particle imaging by improving molecular orientation, enabling clearer 3D structure ...

The approach uses lasers and holograms to detect misalignments as small as 0.017 nanometers. Researchers at the University of ...

Innovative laser alignment methods enable precise imaging of biomolecules, advancing single-particle diffraction techniques ...