The GFP expression constructs were transiently transfected into human kidney 293T or COS-7 cells 7. The amount of GFP protein (P) produced was quantified by western blot using rabbit anti-GFP ...
Purify the vector products by electrophoresis through ... Poor expression of fusions. Check for expression of GFP fusions on SDS-PAGE. Vary concentrations of inducer to obtain roughly ...
“We did something different.” Taking a risk on error-prone PCR, Euskirchen amplified only the GFP coding sequence and inserted it into an expression vector to transform back into E. coli. When ...
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