Substitution of a single amino acid in this helix is sufficient to disrupt SIX1 ... The resulting supernatant was loaded onto an amylose resin column, washed and eluted with 40 mM maltose.

Previously the antisense inhibition of potato SBE B and SBE A as single constructs was reported. The reduction of SBE B (the main tuber branching enzyme) had no effect on the amylose content 9 ...